I work with Resazurin as a anaerobe indicator in gas production system. I found some strange results that I would like to know if these are normal behavior or not?
1- after bubbling buffer for gas production technique with CO2, buffer color changed from pink to colorless for the first time but when I bubbled CO2 again for the third times to the same buffer, its color did not change to colorless. Since chemical reaction between pink and colorless of resazurin is reversible why did this happen?
2- How can clean residual effect of resazurin from bottles? water color change to pink after washing with soap and rinsing several times
What is the composition of your buffer ? I presume that it must contain some reducing agent, since CO2 bubbling may chase dissolved oxygen, but is by itself unable to reduce resazurin to its colorless form. My interpretation is that when you try to perform a third cycle of reduction, the reducing agent that is meant to reduce resazurin is exhausted, thus bubbling an inert gas such as CO2 has no effect any more.
Dear Pierre hanks for your reply.
My buffer has two parts. the 1st part contains:KH2PO4, MgSO4, Nacl, CaCl2, urea, and the 2nd part contains:Na2S, Na2CO3,Cys.HCl and resasorin. according to procedure we should add 20 ml of 2nd part to 1000 ml of first part. I have used this buffer several years ago but I did not have any problems. Today I made two parts of buffer again but after more than 2 hours bubbling it did not convert to colorless.
T
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