I have a sequence of my protein but I don't know whether it has a signal sequence for its extracellular secretion. If it does not, what signal sequence do I need to add for the secretion?
I think we'll need to know what cells you are working with. Secretion signal sequences can vary from species to species - have you had a look at what proteins are known to be secreted by your cells and what kind of signal peptides they carry? That would be a good place to start.
You can check its uniprot id and protein sequence in NCBI. The protein sequence there will tell you how many amino acid long is the proteins primary conformation. If the provided protein sequence is 16aa long than reported length of protein then u may have the signal sequence reported. but in that case the first aa will be Meth and 17 will be meth. rest should match your protein sequence.
This may not be the best explanation you expect but it has worked for me..
Gudluck
My protein is Bovine origin and it is secreted in rare occasions for that it needs some changes in the aa at particular point..i think i have to add a signal sequence to it so that it will be secreted out..i am using pVAX vector and tranfection into BHK-21 cells...
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