Elamurugan Appavoo

6 Questions 6 Answers 0 Followers

Questions related from Elamurugan Appavoo

How can I make a stable HEK293 cell line using pcDNA3.1?

I wish to make stable HEK293 cells with my gene of interest using pcDNA3.1 How can I proceed?

29 December 2014 2,740 18 View

Can someone help with Nitirc oxide estimation?

we are using griess reagent (0.1% naphthylethylenediamine dihydrochloride, and 1% sulphanilamide in 5% phosphoric acid) for estimation of NO. few months before we were getting very good absorbance...

26 December 2014 256 5 View

How does one isolate cytoplasmic and nuclear proteins from cell line (without kits)?

Can anyone suggest a protocol for extraction of nuclear and cytoplasmic proteins from cell line without kits and detergent solution (NP-40)... Can I use Nuclear Extract (NE) buffer (HEPES [20 mM]...

08 September 2014 5,739 6 View

Cloning C-terminal GFP to protein - will this interfere with the expression of GFP?

I have cloned GFP at C-terminal of my protein to confirm its expression by GFP fusion After end of my protein 2 aminoacids are present before start of GFP I wanted to know whether this 2 a.a will...

06 August 2014 6,864 8 View

How can I find the presence of a signal sequence in the proteins that facilitate secretion out of the cell?

I have a sequence of my protein but I don't know whether it has a signal sequence for its extracellular secretion. If it does not, what signal sequence do I need to add for the secretion?

04 December 2013 5,072 5 View

Will eukaryotic protein conserved (differ 2 a.a). mAb raised to one target species work or other target species?

MAb available for mouse protein and I would like to know whether it will work in pig other any other animal?

22 November 2013 9,367 2 View