How can I find the best quencher dye for a reporter in the TaqMan?

28 July 2022 1 6K Report

I am going to optimize a multiplex PCR for 3 different group genes. I will use FAM, HEX, and CY5.5 as reporter. But I do not know how should I find the best quencher for each one. Which reporter is suitable for which quencher?

Farinaz Ziaee

I am going to use FAM, HEX, and CY5.5 as reporter in TaqMan assay.

But I do not know how should I find the best quencher for each one. Which reporter is suitable for which quencher?

Suitable dyes and probes in the instrument that I will use: FAM, SYBR Green, Eva Green, LC Green, Fluorescein, VIC, HEX, TET, Cy3, Cy3.5, JOE, Yellow555, ROX, Texas Red, Cy5, Cy5.5.

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Science method

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