I have only got access to certain cell lines and need to find out which CD markers they express to be able to order certain labels for testing.
Dear Christa
Please find the link https://www.bdbiosciences.com/documents/cd_marker_handbook.pdf
You should be able to find the possible CD markers based on the cell lineage [most of the times]
Dear Christa, the bd list is very useful and a great start but please remember that it is very true what Ravi said that you can rely on it “most of the time”, meaning that you should also use lists from other sources - there is a number of such sources online, almost every company selling CD markers has such list in their catalogue e.g. http://www.ebioscience.com/resources/human-cd-chart.htm and many others. Also, after finding your candidate markers, searching PubMed and Web of Science for specific cell types/CD markers will give you information which are the most reliable markers for a certain cell type and conditions in which cells express those markers and their up-/down-regulation. Good luck with your project.
Hello,
thanks for the links. I am wondering if I can use these lists as follows: I am using for example CHO cells which have been described as being of fibroblast origin. Can I hence assume that they will express at least most of the fibroblast markers? And what would I do with a cell line like HeLa where the origin with respect of tissue as been debated in the literature but mainly given as epithelial (but at least it is a human cell line and I don't run into the issue of crossreactivity) ?
I believe you should also test directly "your own" antibodies in the different cell lines that you may be using. That way you will be sure that your cell line is realy expressing those markers (CDs) and you are not fully trusting the existing Biochemical/Medicine literature.
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