If we want to detect the West Nile Virus by RT-PCR methods, we will need a good and accurate primer for it. I'd like to find a good primer for the 3'UTR region of the virus and use it for my samples and also for my positive control.
Those described by Lanciotti et al. J Clin Microbiol. 2000 Nov;38(11):4066-71 work properly to detect lineage 1 viruses.
Dear Miguel Martín-Acebes,
Thank you for your response and informative guidance,
Regards,
Ali
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