How can I extract human DNA from saliva without using any kit?

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Dietmar Lieckfeldt

Old stuff but works for decades. Feel free to look for some modern adjustments.

A simple salting out procedure for extracting DNA from human nucleated cells.

SA Miller, DD Dykes, HF Polesky - Nucleic Acids Res. Feb 11, 1988; 16(3): 1215.

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC334765/pdf/nar00145-0424.pdf

Saadyeh Rashidi

Many thanks

i used chloroform without phenol. The concentration was around 120 ng. The volume of saliva that i used was 500 ml. It was good