How can I extract human DNA from saliva without using any kit?

More Saadyeh Rashidi's questions See All

Can I extract CEL file information from the TXT file?

Hello, I want to work on some data and I would like to have the CEL file because of its advantages but the article only have provided the TXT file. I would like to know whether it is possible to...

08 September 2017 814 1 View

Hi. Imagine the prevalence of a disease is 20 in 100000, how many sample size should I have to do a case/control study?

I already have 30 sample. I want to also know if we want to analyze the gene polymorphism in rare diseases and we don't have enough sample which other methods rather than case/control study should...

02 March 2017 2,780 4 View

Problem with agarose gel electrophoresis voltage and time. Can anyone help?

I want to run DNA fragments around 200-300 bp. In your opinion, is it ok to use 1.5% agarose, and what do you suggest about voltage-intensity and time? The gel dimension is 8 x 10 cms. I have...

05 June 2014 3,158 14 View

Is there a problem with my RNA pellet?

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11 August 2024 7,082 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

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RNA Extraction Using Hot Borate Method No Longer Working?

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I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

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08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

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Why after performing site directed mutagenesis ,I don't see any colony after transformation?

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Low-yield gel extraction problem?

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Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

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04 August 2024 501 2 View

The question is how to use Wavenet transform?

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Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

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Dietmar Lieckfeldt

Old stuff but works for decades. Feel free to look for some modern adjustments.

A simple salting out procedure for extracting DNA from human nucleated cells.

SA Miller, DD Dykes, HF Polesky - Nucleic Acids Res. Feb 11, 1988; 16(3): 1215.

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC334765/pdf/nar00145-0424.pdf

Saadyeh Rashidi

Many thanks

i used chloroform without phenol. The concentration was around 120 ng. The volume of saliva that i used was 500 ml. It was good