How can I extract DNA by Tris–EDTA buffer and Triton X 100?

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PhD thesis topic?

I want to write a topic for my PhD thesis in hospitality (hotels), can u please suggest some variables

29 July 2024 9,058 3 View

How to converge PEMFC in Ansys Fluent?

I am simulating 1 cell of PEM with an area of 50 cm². The mesh has an orthogonality of 0.2 for the channels and bipoar plates (polyhedra) and a quality of 1 for the active zones (hexagonal). The...

26 June 2024 2,358 0 View

The impact of AI analytical tools?

I am currently conducting a study on the impact of AI analytical tools. Your insights would be incredibly valuable to this research. Would you be willing to spare just a few minutes of your time...

05 April 2024 2,917 1 View

How to apply wavelet transform on real signals from protection relays ?

How to apply wavelet transform on real signals from protection relays ? in matlab simulation the signals are sended to workspace and then the WT is applied but how to apply it to real signals...

02 April 2024 2,653 4 View

What is the role of artificial intelligence in psychological and social crises?

As technology races forward, AI is proving to be a powerful resource in tackling mental health and social issues. Early Warning System: AI can analyze data to predict mental health crises before...

29 March 2024 2,425 2 View

What are the main sport sudden death causes?

Many athletes face sudden death in football or Olympic games without know the causes or misunderstanding to prevent them

27 March 2024 6,257 1 View

How can I get more cloud storage space on my devices, knowing that I have a university email, but my university only gives 100GB?

I don’t have enough space.

27 March 2024 9,707 0 View

Music therapy what is the new?

Dose music therapy still benfit as adjustment management in cancer treatment

26 March 2024 6,122 3 View

What methodologies and technologies are most effective for building interactive chatbots with high responding accuracy?

I am conducting research on the development of interactive chatbots that prioritize accuracy in their responses. My aim is to gain insights into the most effective methodologies and technologies...

17 March 2024 333 3 View

heat stress-chicken ?

Stress has a significant influence on the productivity of poultry, resulting in negative impacts. To reduce these impacts, various procedures and additions have been designed. As researchers, we...

25 February 2024 9,018 0 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I need JCPDS file of LSFCO nanomaterial. Can anyone provide me?

Require for Rietveld refinement in XRD.

08 August 2024 3,081 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

How to understand this crystallographic phenomenon of low temperature crystals in zeolite?

During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...

06 August 2024 726 3 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to fix background error in rietveld refinement of one XRD peak using GSAS-II?

I want to refine one XRD peak of my in-situ xrd but the background is never working good which ultimately fails the refinement. How to refine and adjust the background using GSAS-II

05 August 2024 5,291 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View