How can I evaluate primer complementarity between 6 different primers ?

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Why sometimes I have primer - dimer formation and sometimes I don't ?

Hi Guys, I'm standardazing a conventional PCR, I'm always using the same concentracion of primer and in most of the cases I see what I think its primer dimer formation, but in one sample and in...

09 October 2019 394 7 View

It is possible to contaminate a conventional PCR with a positive Mycoplasma sample ?

I'm running a conventional PCR in order to detect Mycoplasma spp, however i'm having some problems as some negative samples sometimes amplified and I see the band in the gel and sometimes I do not...

11 December 2018 3,794 1 View

Do you know any website or bioinformatic tool that can help me to design Taqman probes for my RTqPCR ?

Hello Guys. I need to design three different Taqman probes for my real time PCR, so I'd like to know if some of you know something about it, any website or tool that I can use Regards.

02 March 2018 5,201 4 View

Do you know how can I improve the purity in my DNA/RNA Extractions ?

I'm making nucleic acid extraction with Maxwell RSC equipment. But the purity 260/280 is not too high. I extract the nucleic acids from plasma samples. So I'd like to know if there's anyway to...

02 March 2018 3,065 4 View

No Template Control Contamination qReal Time PCR ?

I'm getting amplification in the NTC when I run my PCR. I've tried different things as start with new reagents, cleaning the hood and being very careful when pipeting. Can somebody thel me why...

31 December 2017 1,859 3 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Is this website real?

Is this website real? https://isar.org.in/event/registration.php?id=2434532

08 August 2024 484 1 View

U you think We need a website software of Blackbody radiation law expert software?

A website software of Blackbody radiation law expert software can used through the following web site. http://39.105.188.151:3000/index

07 August 2024 1,706 0 View

Gromacs first step of minimization ?

I have face this problem anyone help me how to solve this issue ?which is below Fatal error: There are inconsistent shifts over periodic boundaries in a molecule type consisting of 78 atoms. The...

07 August 2024 2,598 1 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

Is it legal to upload a graphical abstract previously published on your paper's first page to a website such as figshare.com under CC-BY-4.0 license?

Dear friends, does anybody know that is it legal to upload a graphical abstract previously published on your paper's first page to a website such as figshare.com under CC-BY-4.0 license? Thanks.

05 August 2024 7,098 3 View

How can I find access to heavy metal reference doses from the EPA and WHO websites?

Reference dose and Maximum acceptable concentrations HMs

03 August 2024 8,230 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View