Do you know any website or bioinformatic tool that can help me to design Taqman probes for my RTqPCR ?

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No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

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Seied Mehdi Miri

Dear Cristian

I hope the following links and article will be useful to you.

https://www.researchgate.net/post/Primer_design_for_taqman_real_time_PCR2

https://www.genscript.com/tools/real-time-pcr-tagman-primer-design-tool

Badreddine Mechri

Hi Cristian,

I recommend Primer3 free software but you can also use the following on line primer design tools. Best Luck.

http://qpcr.probefinder.com/organism.jsp

http://www.autoprime.de/AutoPrimeWeb

Kevalkumar Shah

You can use Beacon Designer and can be downloaded from http://www.premierbiosoft.com/qpcr/

Abhijeet Singh

Additionally, better contact the manufacturer, they themselves can assistance in designing potent probes.