Dear Cristian

I hope the following links and article will be useful to you.

https://www.researchgate.net/post/Primer_design_for_taqman_real_time_PCR2

https://www.genscript.com/tools/real-time-pcr-tagman-primer-design-tool

Hi Cristian,

I recommend Primer3 free software but you can also use the following on line primer design tools. Best Luck.

http://qpcr.probefinder.com/organism.jsp

http://www.autoprime.de/AutoPrimeWeb

You can use Beacon Designer and can be downloaded from http://www.premierbiosoft.com/qpcr/

Additionally, better contact the manufacturer, they themselves can assistance in designing potent probes.