Cristian Ricaurte

6 Questions 4 Answers 0 Followers

Questions related from Cristian Ricaurte

Why sometimes I have primer - dimer formation and sometimes I don't ?

Hi Guys, I'm standardazing a conventional PCR, I'm always using the same concentracion of primer and in most of the cases I see what I think its primer dimer formation, but in one sample and in...

10 October 2019 374 7 View

It is possible to contaminate a conventional PCR with a positive Mycoplasma sample ?

I'm running a conventional PCR in order to detect Mycoplasma spp, however i'm having some problems as some negative samples sometimes amplified and I see the band in the gel and sometimes I do not...

12 December 2018 3,778 1 View

How can I evaluate primer complementarity between 6 different primers ?

I'm designing RTqPCR and I will use 3 differents sets of primers for 3 different viruses. So I would like to know if there exists complementarity among them. Do you nkow any website or...

04 April 2018 7,007 3 View

Do you know any website or bioinformatic tool that can help me to design Taqman probes for my RTqPCR ?

Hello Guys. I need to design three different Taqman probes for my real time PCR, so I'd like to know if some of you know something about it, any website or tool that I can use Regards.

03 March 2018 5,183 4 View

Do you know how can I improve the purity in my DNA/RNA Extractions ?

I'm making nucleic acid extraction with Maxwell RSC equipment. But the purity 260/280 is not too high. I extract the nucleic acids from plasma samples. So I'd like to know if there's anyway to...

03 March 2018 3,045 4 View

No Template Control Contamination qReal Time PCR ?

I'm getting amplification in the NTC when I run my PCR. I've tried different things as start with new reagents, cleaning the hood and being very careful when pipeting. Can somebody thel me why...

01 January 2018 1,836 3 View