How can I estimate vitamin D level in rat?

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Navneet Ammal Kaidery

I would think I125 RIA wold work. That used to work for me with Thyroid hormone measurements.

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1240027/

Article Determination of vitamin D status by radioimmunoassay with a...

Sakthivel Govindaraj

Is there any normal biochemical methods available to estimate rather than using radio active substance?

Tausif Alam

You may find the following publication in Clinical Chemistry (2012) useful:

State-of-the-art vitamin D assays: a comparison of automated immunoassays with liquid chromatography-tandem mass spectrometry methods

http://www.ncbi.nlm.nih.gov/pubmed/22230812

Leonardo Mendes

Hi. What fraction of the vitamin D you wanted to analyze [25 (OH) D; 1,25 (OH)2D ...]? The best methods are HPLC or RIA (are more sensitive and more specific). Depending on the fraction and the type of study, an alternative would be to measure by ELISA.

Dear Mendes,

Would you please provide estimation by HPLC method.

Hi.

Sorry, but I don't have this protocol...

Best regards

Its okay thank you!!