I am processing high-grade serous ovarian cancer samples and trying to grow tumor cell culture for subsequent functional assays. Any recommendation on the dissociation process, enzyme mix and/or culture media?
https://www.youtube.com/watch?v=4znk90Lg28U
https://www.youtube.com/watch?v=jA9DKdx04Xg
overtrypsinisation has to be avoided. these two videos will help. its almost same as isolating primary culture is what i thought. plus skill.
you can check at www.cellapplications.com
I would search for relevant scientists in the National Cancer Institute. They have created thousands of patient-derived tumor cell lines and they might know a couple of things about that topic.
...in addition to the previous comments following papers may help
doi:10.1038/nprot.2006.328
DOI: 10.1038/ncomms8419
Good luck
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