What is the best way to extract protein form bacterial cells?

Hi Everyone! I have a sample of bacteria cells, and I want to sonicate them to extract cytosolic proteins. I found a protocol using Tris-HCl buffer, but this lab did not use proteases inhibitor....

13 December 2020 2,603 3 View

Soil, water, and air microbiome?

Hi everyone! I am looking for a database that contains a list of organisms, preferentially bacteria, present in the air, water, and soil. Does anyone know any good database/website? Thanks!! Rocio

24 March 2020 2,009 3 View

Why is STZ considered to be inconvenient for type 1 diabetes?

Why is STZ considered to be inconvenient for type 1 diabetes? if when you inject the rats they are not insulin dependent at low doses (45 mg/kg), but if they are hyperglycemic rats. if it were...

19 March 2020 348 4 View

Does anyone know an updated plant pathogen database?

Hi all, I am working on proteomics, and I am looking for a plant pathogen updated database. Most of them are no longer available. Thank you so much!

15 January 2020 7,157 3 View

How education and entrepreneurship is articulated in your country?

In Ecuador, entrepreneurship is in vogue. The country has a legal framework that normalizes the articulation of Education and Entrepreneurship. In addition, Ecuador has the highest rate of Total...

15 July 2019 7,807 6 View

What do you think about the liberation of women in social life?

In these yars wowen are more importat in this sfere of the life

25 February 2019 6,224 6 View

How can muscle weakness of diabetic rats be ruled out if I am going to use the forced swimming test?

to avoid having a bias in the study with diabetic rats, I need to rule out muscle weakness in the forced swimming test or which method I should use to measure the depressive type behavior where...

01 October 2017 270 2 View

How to perform a Statistical Analysis?

12 September 2017 4,206 12 View

TaqMan MicroRNA cell-to-Ct Kit?

Hi! It is my first time using TaqMan kits, so want to know something about Reverse Transcription process. I recently bought this Kit to extract microRNAs, to do the RT and to quantify on qPCR, and...

19 April 2017 5,157 2 View

Has anyone use anti-FLAG antibody for immunohistochemistry in paraffin/cryostat sections?

27 October 2016 3,966 1 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

How to model Supercritical water gasification (SCWG) process in Homer software?

Hi everyone How to model Supercritical water gasification (SCWG) process in Homer software?

25 February 2021 4,075 1 View

How to constrain genetic algorithm input u?

My real system (buck converter) can only take an input of 0 to 1 (duty ratio) and I need to constrain the system so the controller action keeps within this bounds. How can I do this? The code for...

22 February 2021 2,125 3 View

Any suggestions or recommendations on a good publishing house based on the African for academic books in the field of Communication and media studies?

I am currently working on a book on Public Relations (from an African perspective) and the rationale behind the book is the fact that as instructors in this field, we borrow instances and case...

19 February 2021 2,482 2 View

When to use different framework?

Hi, can I use both conceptual framework and theoretical framework in my systematic review? My future study plan is to use a theory. In this situation for my systematic review should I use both or...

18 February 2021 9,581 2 View

Person-Codes for questionnaires in longitudinal studies: Is there an easy solution for better matching-rates?

Hi, I have a problem regarding matching of longitudinal data in my field of research (university students). We have a Person-Code for matching data measured in different time points, but 30%+ of...

17 February 2021 3,075 4 View

Can anyone provide an advice on running a validation study of a commercially available product in collaboration with the manufacturer?

We are planning to run a study validating a commercially available product (i.e. software) and while we initially planned to do it independently of the manufacturer, they heard about our intention...

17 February 2021 2,295 3 View

Choice of Ab for Co-IP: Does an anti-(tag) antibody (such as anti-flag/ anti-myc) a must if I am using co-transfected cells for Co-IP?

Hi guys, I am trying to detect the binding of 2 proteins by Co-immunoprecipitation. However, the choice of antibody is confusing me. I tried probing the endogenous protein right away but the...

16 February 2021 9,050 5 View

Is Genetic Algorithm usage for Motor Conception FEA optimization a good choice?

Hi RG community, Like the title shows ,i was wondering if choosing the GA for this kind of Optimizaiton is a good choice? For example we have a population of 100 (for 7-8 Optimization Variables),...

14 February 2021 1,160 7 View

What can explain a low expected heterozygosity rate for T. monococcum wild samples (0.09) ?

I conducted an SDS PAGE electrophoresis on wheat wild varieties to separate their glutenins and measure the genetic diversity of my sample. I only ran one electrophoresis. Can a genetic drift...

14 February 2021 3,971 1 View