I tried doing colony formation assay of treated cancer cells using 0.5% crystal violet in methanol (v/v). But colonies are not formed at the end of experiment. Please any suggestions?
Dear Mahmood Hassan Dalhat please see this article entitled "Clonogenic assay of cells". The paper is available as public full tex on RG:
Article Clonogenic assay of cells
The following article entitled "High-Throughput Fluorescent Colony Formation Assay" is freely available as full text on the internet:
https://www.biotek.com/assets/tech_resources/High-Throuput%20Fluorescent%20Colony%20Formation_App_Note_Final.pdf
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