How can I do colony formation assay using the Crystal Violet method?

11 January 2021 1 8K Report

I tried doing colony formation assay of treated cancer cells using 0.5% crystal violet in methanol (v/v). But colonies are not formed at the end of experiment. Please any suggestions?

Frank T. Edelmann

Dear Mahmood Hassan Dalhat please see this article entitled "Clonogenic assay of cells". The paper is available as public full tex on RG:

Article Clonogenic assay of cells

The following article entitled "High-Throughput Fluorescent Colony Formation Assay" is freely available as full text on the internet:

https://www.biotek.com/assets/tech_resources/High-Throuput%20Fluorescent%20Colony%20Formation_App_Note_Final.pdf

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