As I am working with Chlorella vulgaris stain. During fluorescence microscopy analysis the cells were showing little fluorescent intact and same time floating under the microscope that I am unable to take images.
kindly help me out
Dear Priti,
Please find attached articles related to Nile red staining. We are using same protocol. Hope it will work for you. Though I prefer BODIPY dye instead of Nile red.
About little fluorescence, the use of solvent for dissolving nile red dye, the incubation time and temperature is important. I have attached a link hope it might help you.
http://www.sciencedirect.com/science/article/pii/S0167701209000037
Thanks poonam for providing me good paper and answer to my query i will try it once again
@ ramdhony thanks for your valuable answer and I am using 40 % DMSO and with 10 minute incubation time and 40 degree temperature.
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