Hello,

you can do this very easily with Genalex: http://biology-assets.anu.edu.au/GenAlEx/Welcome.html

this software is very user-friendly, and it works as an extension of Excel.

Hope this can help you.

You can simply get a PCA from a microsatellite genepop format using GENETIX in the AFC selection - it's in french but its easy to use and you can re-graph results in R if needed.

http://kimura.univ-montp2.fr/genetix/

Hi Paulo

Or you can go directly into R and use the gstudio package (among others). Here http://dyerlab.github.io/gstudio/ you have a nice tutorial on how to use it

Hope this helps

Pablo

You can also use adegenet package in R (https://cran.r-project.org/web/packages/adegenet/index.html, see also https://github.com/thibautjombart/adegenet/wiki for tutorials).