I have tried using water, acetonitrile, methanol, DMSO and their combinations with no results for concentrations arround 15 mg/mL. I need to use them as standards for amino acid analysis by HPLC or mass spectrometry.
Thanks
I would suggest to check the solubility from producer information or Merck Index. Some of them are soluble in acidic and others in basic environment. warming and mixing is also necessary. Some, eg Cys are better soluble if in the form of salt - Cys-HCl.
The solubility in a given solvent is different for different amino acids. Some of them are well soluble in water. For others you can use DMF. You can use amino acid hydrochlorides as standards - they are well soluble in water. If you do not have them - add some HCl to the suspension of amino acid in water. Na salts may be also soluble - you can just make your suspension basic.
How to make amino acids soluble in distill water while using it for yeast growth media,? HCl, DMF or other solvent will effect the yeast growth or no,,? can we autoclave amino acids along with the growth media at 121 °C ,? 121 °C will effect amino acid nature or no,? Prof. Igor Dubey, Prof Gaetan Glauser, etc all please help
I just decreased the concentrations to 5 mM and dissolved them in 0.1M HCl, some of them needed some shaking and only cistine (cys-cys) needed ultrasound, Thanks for your answers and sorry for taking so long to give you a feed back.
How to avoid amino acid precipitation, after mixing and dissolving with HCl or NaOH. After some time the amino acid start precipitation?
Dear Orlando, u try either with 1M NH5CO3, or 3N HCl or 30% liquid ammonia or 5 M NaoH for solubility of all amino acids, and I m pretty sure all amino acids (11 amino acids) except Leucine, Isoleucine, Phenylalanine, Aspartic acid, glutamic acid will easily disolved in water.
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