What is the best suited direct protocol for miRNA isolation from exsosome?

I want to isolate exosomal miRNA only, for that do I need to first isolate total RNA and then go for miRNA isolation? or can I directly isolate miRNA from exosome and do the analysis? if yes...

08 December 2020 5,803 4 View

What potential ethical issues arise due to the way technology has transformed the field of education?

Distance education over the Internet and online examinations are a great way to impart and assess education across the globe to those who need it and cannot make it to full-time campus...

15 July 2019 142 3 View

How to dilute human Fc block for immunofluorescence?

Hello guys, I am a BSc student and my supervisor is currently on holiday and I forgot to ask about this. i have a human FC block from BD Pharmingen. It does not state the dilution i need to do for...

19 June 2016 4,827 3 View

Help with Western Blot technique?

Hello everyone, I am currently doing my research project for my final year of degree and I don't know how to go about the western blot. I have never done this technique before and my supervisor...

08 April 2016 7,601 2 View

Why does our diagnostic double digest of pGL4.27 plasmid yield bands that are longer than expected?

We performed an overnight double digest of pGL4.27 plasmid using XhoI and BamHI in a 37oC water bath. After running the digest on a gel along with a 2log ladder, we observed two bands: one around...

06 October 2015 3,267 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

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01 March 2021 3,905 5 View

What test should I use to compare two or three means, if data is not normally distributed but sample size is > 30 in each group?

I personally feel this as a most confusing question. As per my understanding, if sample size is > 30, and sample is skewed, we can still use paramteric approach, as it will follow Z...

01 March 2021 1,587 6 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Sample size determination for non-parametric tests?

How do I run a a priori sample size determination for non-parametric tests, like the Mann-Whitney U-test and the Kruskall-Wallis or other non-parametric tests that accord with the unpaired t-test...

24 February 2021 166 6 View

How to determine a priori sample size for t-test with unequal means?

I need to conduct a a priori sample size determinations for t-test with unequal means, but I don't know the sizes of the groups nor the ratio of both groups... Can I do a sample size determination...

24 February 2021 8,289 3 View

What are alternatives to factor analysis for a small sample?

My sample size is too small to conduct a factor analysis. I created my own psychometric scale for measuring Effectiveness of Support, which contains 38 items (4 sub-scales) Im really struggling...

23 February 2021 9,287 12 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View