Can we use PAGE to detect presence of antibodies in plasma? I am planning to characterize antibodies after immunization of mice with whole venom. How can I purify the plasma to get rid of other unnecessary protein components like albumin and others.
Do ELISA or western blot to detect the antibody. SDS-PAGE of crude plasma may give you many bands in addition to the antibody. You can undertake chromatography (size exclusion and affinity chromatography) to purify the antibody.