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Questions related from Ren Vien Anthony Fernandez
Can anyone suggest a good method for the preparation of naphthaldehyde dimethyl acetal? I have been looking for chemical vendors that might be selling it but couldn’t find one. Other methods I...
15 June 2023 482 0 View
I am trying to perform a silylation reaction and need to find a way to quench the excess TMSCl. I am particularly concerned with the evolution of white vapor after the completion of my reaction.
16 March 2023 566 0 View
I intend to dilute my protein samples in PBS but am concerned since this is a different buffer system compared to the Tris buffers used in PAGE. I would like to ask if suspending my proteins in...
12 June 2021 1,861 6 View
I am doing SDS-PAGE using an 8% resolving gel and 5% stacking gel with a Tris-glycine running buffer. During electrophoresis, I get a smeared/tailing dye front which is totally opposite with the...
07 May 2021 2,311 3 View
We are trying to elute native protein bands by passive elution due to unavailability of an electroelution device. How can the gel be preserved if the gels cannot be used for elution immediately?
13 April 2021 9,438 4 View
Can we use PAGE to detect presence of antibodies in plasma? I am planning to characterize antibodies after immunization of mice with whole venom. How can I purify the plasma to get rid of other...
31 July 2020 3,512 1 View
Can someone help me on increasing the number of geometry optimization cycles in ORCA. I already adjusted the number of SCF iterations per cycle.
19 March 2020 1,679 3 View
Hello! I have conducted a series of experiments for drug binding of a certain compound to HSA (serum albumin) such as fluorescence, UV-Vis, etc. Is it alright to publish my results without an...
10 October 2017 7,041 5 View
What algorithm does Autodock Vina use? I have read that it uses LGA but I need confirmation. Also, I would like to know if it is valid to first do a blind docking, and find the site with the most...
24 January 2017 1,939 6 View
What are the latest advances in chimeric/artificial/synthetic cell receptors and who are the leading researchers in this field.
22 January 2017 7,229 0 View
