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Questions related from Ren Vien Anthony Fernandez
I am trying to perform a silylation reaction and need to find a way to quench the excess TMSCl. I am particularly concerned with the evolution of white vapor after the completion of my reaction.
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I intend to dilute my protein samples in PBS but am concerned since this is a different buffer system compared to the Tris buffers used in PAGE. I would like to ask if suspending my proteins in...
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I am doing SDS-PAGE using an 8% resolving gel and 5% stacking gel with a Tris-glycine running buffer. During electrophoresis, I get a smeared/tailing dye front which is totally opposite with the...
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We are trying to elute native protein bands by passive elution due to unavailability of an electroelution device. How can the gel be preserved if the gels cannot be used for elution immediately?
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Can we use PAGE to detect presence of antibodies in plasma? I am planning to characterize antibodies after immunization of mice with whole venom. How can I purify the plasma to get rid of other...
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Can someone help me on increasing the number of geometry optimization cycles in ORCA. I already adjusted the number of SCF iterations per cycle.
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Hello! I have conducted a series of experiments for drug binding of a certain compound to HSA (serum albumin) such as fluorescence, UV-Vis, etc. Is it alright to publish my results without an...
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