Hi.
I'm trying to detect the phenol red in the mixture solution by HPLC.
As you know, It is using for ph indicator.
The problem is..
After making phenol red solution in 50 ml conical tube, I transfer it into Agilent glass vial for HPLC or Shimazu vial.
But as soon as I transfer it, the color and pH were changed.
Is there someone who suffered the same problem as us?
Or is there any way to detect it with a fluorescent detector??
Thank you :D