I try many times to do it
Your research based on Temperature Only. Hence you verify your guides for suitable temperature.
Does anyone know of an appropriate solvent to dissolve magnesium oleate? I have tried searching the literature and I am seeing that the salt is insoluble in many solvents. I have tried water,...
14 October 2022 2,884 1 View
Soccer is a game played by two teams of eleven players with a round ball that may not be touched with the hands or arms during play except by the goalkeepers. The object of the game is to score...
02 January 2021 2,890 90 View
If they are more than one thing you may mention them, please. That could help others to learn from your experience. Let us learn from each other.
29 December 2020 1,012 93 View
Writing a good paper or article considered to be a great effort in order to reach incredible results. Hence, which style do you think is more popular and better to be used? Is it MLA Style,...
26 December 2020 339 20 View
Academic writing should be well designed. How can we be professional? What are the main tips though?
26 December 2020 1,162 7 View
I am working on a reductive amination conjugation reaction between a tag I've synthesized and a glycan (I am using lactose to test out the protocol). Total molecular weight of the conjugate is 689...
17 December 2020 8,430 3 View
16 December 2020 4,409 0 View
I have coupled a reagent to lactose, followed by a reductive amination reaction with sodium cyanoborohydride in a ~100 uL solution. I want to remove the NaBH3CN from solution. What is (are) the...
01 June 2020 3,859 6 View
To evaluate the quality of feed
24 November 2014 8,095 6 View
Ellipses:- What is an ellipsis? An ellipsis is a series of three consecutive periods known as ellipsis points ( . . . ) used to indicate where words have been omitted from quoted text, or...
01 January 1970 5,822 9 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks
06 August 2024 949 0 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
How to design VN primer to attach with universal reverse primer
05 August 2024 2,116 3 View
Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...
01 August 2024 4,606 7 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...
01 August 2024 4,673 4 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View