How can i design a primer for specific traits?

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How to make overlapping p-h diagram in EES?

I want to draw ph diagram using Engineering equation solver EES for refrigerant R134a and R1234fy and some other. I don't know how to draw the multiple ph diagram on same dome or overlapping to...

30 July 2024 647 7 View

How to we see transcription activity of the bhlh79 family is at the N-terminal or C-terminal?

I'm a PhD Student. From Northwest Agriculture and Forestry University China. My Problem is this I start my work on bhlh79 transcription factor gene. I do my Y2H 5 time but the colonies appear on...

30 July 2024 1,412 8 View

How to determine the quantity/amount of secondary phase based only on peak intensity?

I have 3 different specimens. Each specimen has a main peak of the targeted phase, however, all of them also have a secondary peak of associated with a secondary phase. Now just based on the...

26 June 2024 5,049 5 View

How can machine learning be employed in the field of natural sciences?

Hello! I am pursuing Ph.D. in Environmental Sciences from Sambalpur University, India. My thesis topic broadly focuses on physico-chemical analysis of water and soil samples. I eagerly want to...

18 June 2024 1,897 4 View

Feeding zebrafish larvae properly, so how many grams or milligrams of Artemia should be given to 6 dpf larvae?

According to standard guidelines, experts suggest proper feeding ratios to grow the ZF healthly.

15 June 2024 4,354 1 View

How can I begin quantum computing on my computer or laptop?

I am interested in designing, developing, and testing algorithms on my laptop or local machine. Do I require any specialized quantum hardware or an online quantum computing service? Is it possible...

10 June 2024 2,917 3 View

Normality test of DV for multiple regression is violated. Can I still carry on with multiple regression? How can I report it?

I carried out a normality test on my DV and turns out it is significant hence violating the assumption. Do I carry on with multiple regression or what else can be done in this case?

30 May 2024 963 2 View

How to conduct comparative study of refrigerants with one heat pump model as they have different saturation pressure and temperature?

I have modelled a refrigeration system using finned and tube heat exchanger and adopted the tube by tube method. I chose the R134a as a baseline refrigerant and developed the model according to...

30 May 2024 8,152 12 View

How are emerging technologies reshaping the financial industry on a global scale?

Emerging technologies are fundamentally changing the way the financial industry operates on a global scale. How two of the most impactful ones, artificial intelligence (AI) and blockchain are...

28 May 2024 3,009 4 View

Can someone help me with the alpha amylase inhibition assay?

I am determining the alpha amylase inhibition activity of my methanolic extracts by DNSA method. The problem is, my sample absorbance is higher than the negative control which made the final...

27 May 2024 9,348 0 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Research Methodology - Impact of Corporate Reputation on Stakeholders Behaviors?

Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks

06 August 2024 949 0 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

Should I remove an item from a scale to raise Cronbach's alpha and McDonald's omega or is it better to leave it if they are both over .7 already?

Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...

01 August 2024 4,606 7 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...

01 August 2024 4,673 4 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

How to retain amplicon yield after Ampure bead cleanup, following a 2-stage PCR protocol?

Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...

30 July 2024 841 2 View

How to Input and Analyze Results for an Asymmetrical Factorial Design in STATISTICA 13.3?

In STATISTICA 13.3, I used the Experimental Design Builder under the Design of Experiments tab to generate a design matrix for an asymmetrical factorial design (1443) with 12 experiments. However,...

29 July 2024 4,155 2 View

Pragmatic inquiry research design?

Employing a pragmatic inquiry research design, looking for published research using this method, employing qualitative research data collection methods of semi-structured interview and focus...

28 July 2024 540 2 View