How can i design a a primer for a specific gene which is not recorded before (not in ncbi )?

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Karen A. Darbinyan Popular answer

Hello, if you have sequence of your gene of interest you can easily design primer by any of primer design program.

Karen A. Darbinyan

Annemarie Honegger

You may look at a multi-sequence alignment of the orthologous genes in related species. Regions that are highly conserved in this alignment are very likely also conserved in the species you are interested in, and primers directed against these regions therefore likely to also recognize the gene in your species of interest.