Hey there!
I have performed a quantitative proteomic analysis using TMT labelling and I have the results in an excel file as pairwise comparisons of every identified protein. Proteins were assumed to be differentially up or down regulated when log2FC was equal or higher than 1 in absolute values and the FDR was equal or lower than 0.05. I have 4 groups that have been compared all pairwise, and I will like to create a Venn diagram showing the proteins that are not differentially expressed in all of the groups, the proteins differentially expressed in one group vs. the others, etc...
Any advice on how can I proceed in order to create the different lists of proteins that I will need for the creation of a Venn diagram including all the comparisons between the 4 groups of my experiment?
Thanks a lot in in advance!