Is anyone familiar with creating an in vitro autoimmune model using any human epithelial cells?
Hi Esther Nutifafa Senanu
My suggestion would be to work along the lines of a paper from Kragstrup and colleagues published in 2019.
Article IL-12/IL-23p40 identified as a downstream target of apremila...
Even though mesenchymal synoviocytes display a lot of properties of epithelial cells in monolayer/ 2D cultures (while lacking desmosomes/ tight junctions). To that end I would concentrate on a cell culture model for psoriasis for which you have culture models available that are summarized here:
Article In Vitro Disease Models for Understanding Psoriasis and Atop...
You can even set up models for ultra-rare diseases like epidermolysis bullosa, which while not autoimmune diseases in the classical sense, they display a barrier dysfunction that subsequently leads to a strong inflammatory component, in more detail described here:
Chapter Isolation and Culture of Epidermolysis Bullosa Cells and Org...
In general, broad targeted analysis of the proteome (like in the Kragstrup paper) should help with the analysis of these (depending on your experimental choice) increasingly complex models.
I hope that helps!
Good luck experimenting,
Michael