I have isolated total RNA from a yeast by using a kit, but the concentration is not very high. How can I concentrate my existing RNA samples?
You can concentrate the RNA by precipitation with glycogen, which is available from several companies, such as SIGMA or Invitrogen
Through speed vac with low temperature, you can also concentrate your RNA samples.
You can use vacuum concentrator at a tempreture of 30 degree celcius for 15min. Place the RNA on ice for 10- 20 min and then return it back to the concentrator for another 15 min. The concentration should substantially increase.
Alternatively, you can preheat the elution buffer at 50-55 degree for like 20 min and immediately use it to elute your RNA. Normally this practice gives me better RNA yeild.
Hope this helps
Bashir
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