How can I compare and analyze DEGs from two samples with different statistical significance?

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Phospholipid metabolism microarray?

Any suggestion on microarrays (protein/ DNA/ RNA) that measure enzymes in the phospholipid metabolism pathways? Thank you.

18 December 2023 1,366 3 View

what are the best approaches applied ?

Plastic waste is becoming a challenge in managing and people are enjoying using it with its less expense, so solution with simples deal is very crucial.

01 December 2023 860 2 View

How to carry out inter-rater reliability assessment in a retrospective chart review study?

I am planning a retrospective chart review study using data abstraction form. My questions are: 1) How many number of cases/percentage of cases to be selected for inter-rater reliability...

14 September 2023 7,448 0 View

How can i determine surface area of TiO2 particles on the CNF?

I made TiO2/ACNF. It are TiO2 nanoparticles with an average size of 10nm on an Activated carbon nanofiber (ACNF). Also, TiO2 are embedded in the ACNF. I conducted BET, CV and Aicd Titration...

06 September 2023 4,083 2 View

My Articles are updated to Another person?

Hello My name is Do-Hyung Kim, from South Korea. I work at Samsung Changwon Hospital. My name is first name and last name is common in South Korea. I want to update my articles, but since...

05 August 2023 7,511 6 View

How to determine the equivalence margin to study safety of biosimilar?

I am planning a study to examine the safety of biosimilar compare to reference product. Efficacy of biosimilars are usually compared using equivalence trial design, however I couldn't find any...

27 May 2023 8,650 1 View

Any open access of phytoplankton functional types database?

Hi all, I'm looking for PFT time series (global scale preferred). I found OSS2015 PFT (PHYSAT) in hermes but I wonder is there any other open database of global PFT to compare with earth system...

01 December 2022 7,515 3 View

Is it possible to run multilevel EFA with R?

I intend to analyze a data set with multilevel EFA, which will provide me with within and between loadings. Can anyone share with me, if there is any R package that can run multilevel EFA? Thank you.

04 September 2022 4,799 0 View

Is there any difference in the binding affinity of Fc-silent antibodies to FcRn and Fc gamma receptor?

Hi, I have a question about the binding affinity of Fc receptors including FcRn. It is known that Fc-silent antibody does not bind to Fc gamma receptor. However, I wonder if it does not bind to...

17 February 2022 6,874 1 View

Why do my primary neuron cultures look swollen after ICC?

I am currently working on setting up primary neuron culture in the lab. I use E18 Rat or E16.5 Mouse. I use HBSS supplemented with HEPES and P/S for dissecting buffer. I use a Complete Neurobasal...

29 August 2021 8,546 2 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View