what are the best approaches applied ?

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Confused primer dimer or something else?

Hi everyone, i recently did a qPCR to check the best annealing temperature for my primers. So i ran two of my primers on annealing temperature 59C with a melting curve. The melting curve shows...

17 November 2020 2,187 5 View

RNA integrity with agarose gel?

Hi everyone, I just recently did some RNA extraction for my insects sample for RNA-seq. I have checked both the concentration (average 450ng/uL) and 260/280( ~2.0). I also done a agarose gel run...

21 October 2020 1,915 3 View

How can I compare and analyze DEGs from two samples with different statistical significance?

I'd like to compare and analyze DEGs (differentially expressed genes) of PBMC from two different organisms (A, B) under hypoxia. I got more than a thousand DEGs from A that meet p

19 August 2020 3,847 2 View

Bi-sulfite primer design for wg gene in pea aphid?

Hi all i am looking to design primer for the wingless gene in pea aphid (wnt-1) protein to look at the methylation status of the gene. Since i dont know where the methylation point is in the gene...

27 February 2019 6,238 11 View

Designing primer for a gene for DNA methylation purpose?

I need to known a DNA sequence for a gene in the plant(V.faba) in order to design primer for PCR for DNA methylation purpose analysis.However I only had the mRNA seq and AA seq for the gene in...

18 October 2018 3,674 4 View

Bacterial total RNA extraction (including small RNAs) using mirVana renders only molecules under 200nt???

I am trying to extract total RNA from bacteria (Salmonella) using the mirVana kit. I am interested in studying the total trasncriptome, inlcuding small RNAs. But after extraction (I elute in...

21 December 2017 7,054 6 View

What is the best method in designing managed landfill?

Open dump is the worse, sanitary landfill is the best but impossible for developing countries so managed landfill is appropriate both technical and finance.

31 October 2017 3,983 4 View

Composite reliability more than 0.9 - desirable or undesirable?

In determining internal consistency reliability for measurement models, composite reliability values of more than 0.9 is deemed not desirable because this indicate "they are measuring the same...

12 October 2017 1,198 10 View

Could you share experiences on the good raw materials for composting and methane fermentation?

I am interesting in making compost, especially for farmers. Composting and metahne fermentation are the good way to reduce organic waste to landfill and good for incineration in terms of energy...

01 January 2015 5,556 5 View

Can anyone recommend methods to restore ponds from eutrophication by simple way?

Eutrophication happens in hot climate, especially with high concentrations of nutrients. Algae always cover on the surface of water which protect the light from the sun causing a lack of of oxygen...

15 October 2014 2,983 20 View

Please explain how the plastic input value should be considered from the true stress-strain curve for the bilinear elastoplastic material model ?

I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...

05 August 2024 454 3 View

Water Consumption in Plastic Recycling: Is it Higher Than for Virgin Plastics?

Hello, dear colleagues, I would like your help in finding a reference that provides an average water savings due to plastic recycling compared to the consumption of virgin materials, preferably...

29 July 2024 4,630 0 View

How to dispose off lipids waste?

I am looking for a lipid waste disposal method, keeping in mind the environmental, health and safety aspect of lipid waste. Could someone please provide guidelines or the lowest acceptable...

25 July 2024 637 5 View

Interpreting ATR-FTIR Spectra for Microplastics Research?

Hello everyone, I am currently pursuing a PhD in Environmental Engineering with a focus on microplastic studies. My background is in Civil Engineering, and I am relatively new to working with...

18 July 2024 7,258 3 View

What are the effects of coffee husks waste to the environment ?

Coffee waste

16 July 2024 1,303 3 View

Why am I seeing only one initial maximum in torque-time curves for the fusion of PVC in a Brabender?

We're studying the stability and fusion behavior of multiple PVC compounds in a Brabender. After a few trials, we set the temperature at 195 °C and the rotor speed at 70 rpm. Samples differ in...

12 July 2024 2,768 1 View

Quelles applications innovantes peuvent bénéficier des propriétés des géopolymère?

Les géopolymères offrent plusieurs propriétés intéressantes qui peuvent être exploitées dans diverses applications innovantes : 1. Construction durable : - Bétons à faible empreinte carbone -...

10 July 2024 7,457 1 View

What are the latest advances in the use of geopolymers for nuclear waste storage?

Geopolymers have gained attention for their potential in immobilizing radioactive waste. Recent studies indicate that they offer a promising alternative to traditional cement binders. Geopolymers,...

06 July 2024 9,797 0 View

What are possible reasons for having the repeated wavy patter in Raman spectrum?

I'm currently analyzing the Raman spectra of coloured plastic samples and have encountered a wavy, oscillating pattern in the spectra of some coloured samples. I was wondering what the possible...

04 July 2024 1,949 4 View

How validate the dynamic molecualr simulation with experimental data?

I used forcite module of Material Studio software to simulate the dissolution of zinc production waste in deep eutectic solvent. UFF force field has been used for the optimization of molecules,...

02 July 2024 7,494 1 View