How to determine the ratio of bacteria spore and food pellet as a feed for shrimp? Example, 2x106 spores per 1g of food stated in First Litopenaeus vannamei WSSV 100% oral vaccination
protection using CotC::Vp26 fusion protein displayed on
Bacillus subtilis spores surface (Valdez et al., 2014)
When working with Bacillus spp. it can be mixed directly into feed pellets because it withstands high temperatures \ (up to- 60 ° C. To confirm the bacterial concentration should use Tryptona Soya Agar (TSA) medium with 2,5 % NaCl.
do the total plate count of the bacteria and directly mix along with the water used for feed preparation
Plate count is useless because spores clump. Using plate counts you will tend to underestimate the amount of spores.
Thanks for your answer. I will do plate count after i harvest the bacterial spores and undergoes about 5 cycle of centrifuge, wash and vortexing process. I wonder why plate count is useless ? what if i take small sample of the sample and dilute it with higher dilution factor ?
It is useless because the spores are charged and they aggregate. I can send you methodology that will allow you to more accurately count them if you want to send me your e-mail address. Mine is [email protected].
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