Hi everyone,
I'm seeding primary mouse stem cells into my PDMS-based organ on a chip device. However, the cells do not stick. We have tried coating the chip using 1: 100 and 1:20 Collagen, 2% matrigel, 1:100 poly d lysine so far but none of them worked and the cells still do not stick to the chip.
I was wondering if anyone has had experience with primary mouse stem cells in microfluidic chips and can help me out.
Thanks