No streamlines present in a small region inside a flow. This means exactly what?

No streamlines present means whether the flow is getting obstructed there and the velocity inside the region is zero? Or else, the fluid is trapped inside that region with a nonzero velocity?...

22 February 2021 5,249 10 View

How to polish the samples which are very prone to oxidation for metallography?

I am trying to polish the rare earth based sample for SEM analysis. But the sample is getting oxidized immediately . Can anyone suggest a way to polish these kind of samples?

27 January 2021 4,699 2 View

How do we solve optimization problems involving a maxima or minima in the objective function ?

I am trying to solve a multi-objective optimization problem using the Pyomo library in Python. The objective function includes operational optimization of an electrolyzer for hydrogen production...

11 January 2021 8,018 3 View

Any researchers working on Mucuna pruriens with respect to tissue culture?

15 November 2020 8,675 1 View

How can I confirm that a gene is downregulated in cancer cells through PCR?

A particular gene (gene A) is reported to be downregulated in cancer cells as compared to the normal cells. How can you prove this with the help of PCR?

27 September 2020 5,292 4 View

Why my absorbance values are in negative for antioxidant assay by dpph method ?

plant extract stock is 10 mg/mL from which I pipetted 20, 40, 60, 80, 100, 200 uL and made up to 3 mL using methanol then i added 1 ml of 0.004% dpph solution (dissolved in methanol) incubated in...

02 September 2020 1,218 2 View

Is 5ug/ml puomycin works for Huh7 cell lines?

I am trying to make KO in Huh7 cell line but after transduction even at 5ug/ml concentration Huh7-Ctrl cell haven't died. Could someone please recommend me the working concentration of Puromycin...

17 August 2020 5,427 2 View

How can I find the relation of cancer stem cells and tumor microenvironment?

in Gastric cancer both cancer stem cells and tumor microenvironment prevents the action of therapeutics and drugs. a. How can I find a co-relation between them? b. How can i evaluate their role...

06 May 2020 6,428 7 View

Can anyone please suggest me any book or chapter on Light Stimulated Chemical Kinetics and Mechanism from recent publication?

I haven't found any useful resource until now. The broader domain is Photo-Chemistry.

22 February 2020 6,642 3 View

What should be the best Performance Metrics for SVR Model ?

I am getting the following results for my SVR Model using Python's Sci-kit library : SVR RESULTS - C AND GAMMA PARAMETERS OPTIMIZED BY GRID SEARCH RMSE = 0.17050234208788798 MSE =...

08 February 2020 6,017 2 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

What is the best solvent to remove PDMS from nano particles?

I want to remove PDMS oligomers from the surface of nanolayers. I have just read a very helpful paper about the compatibility of PDMS with different solvents( Solvent Compatibility of...

24 February 2021 7,845 5 View

How to best wash a 96-well plate containing microplastic?

I am planning my protocol for my dissertation for an experiment quantifying bacterial (E. coli) biofilm formation on micro-plastic (5mm discs of polyethylene bags) by conducting a crystal violet...

23 February 2021 7,868 2 View

How to clean up TEM grid with plasma cleaner?

I need to clean the hydrocarbon contamination on the carbon substrate of TEM grid. However, I heard that substrate could be broken by the powerful plasma. My substrate is pure carbon, with ~3nm,...

23 February 2021 3,262 5 View

How can I improve the concentration of banana fruits extracted RNA?

Currently, I'm working with banana fruits. I've tried to do RNA extractions with different methods, and I've gotten total RNA without contamination, but I couldn't get a high concentration of...

22 February 2021 6,126 2 View

Is there a way to grow biofilm in lab to mimic biofilms in showers and baths? Also, how can I measure it?

Hello, I am new to biofilm research and have a question about growing and measuring biofilms. We want to research the effect of a type of enzymes in breaking down biofilms in showers, baths, hot...

14 February 2021 3,522 3 View

3T3-L1 cells, is this a bacterial contamination or a cross contamination with different cell line? or something else?

Hi, I am experiencing some difficulties with 3T3-L1 cells culturing recently. I am working with 3T3-L1 cells for almost 4 years now and have seen bacterial contaminations/ unhealthy cells and...

13 February 2021 4,040 3 View

What is the method of removing RNase contamination from a filter paper surface?

I am working on a biosensing project on paper substrate, however, it is found that the RNase contamination occurs on the filter paper. Might I ask is there any method to clean the paper surface...

12 February 2021 3,926 3 View

How do I stop losing peak shape with Waters T3 Atlantis Column?

I am using this column with a gradient of acetonitrile vs a phosphate buffer. I clean the column after every run with 2%ACN:98%H20 and store in 30% ACN. I've noticed that my peaks are getting...

10 February 2021 9,181 1 View

How to prepare OH- terminated Si Wafer?

Hi, Folks. I am working on the ALD project to deposit Al2O3 on the Si wafer from TMA and Water. I have struggled with the preparation of OH- terminated Si wafer. I have both Si(1 0 0) and...

08 February 2021 8,792 2 View