Number of cells for extacellular vesicles extraction?

hello! I want to extract vesicles from MDA MB 231 cell ligne, culture in serum free media for 48h, would you help me with th number of cells required to have the vesicles? thank you

03 December 2020 6,488 2 View

What analyzes can we do to characterize and know the different composition of a seed oil?

What analyzes can we do to characterize and know the different composition of a seed oil? which devices can be used? Thank you

26 January 2020 1,927 2 View

What are the criteria of academic promotion in your university/country?

How many requirement should be met to get the academic promotion in your university. number of publications for example.

30 May 2019 7,909 7 View

Analysis for additive, antagonistic, or synergistic interaction?

I would like to calculate the expected mortality using the formula of Nishimatsu and Jackson (1998) Pe= Po + (1- Po) (P1) + (1 - Po) (1- P1) (P2) with Po is the natural (control) mortality, P1 is...

07 November 2018 4,017 3 View

What is the best software to perform Whole Genome Sequencing?

Hi ! In my lab, we plan to do Whole Genome Sequencing (WGS), can someone tell me the best dedicated software for this work? I thought I understood that these software require having super...

23 May 2017 5,439 3 View

Can someone explain me what's the difference between reactogenicity and the immunogenicity of a vaccine?

Can we evaluate-it on animals, or only in humans?

09 March 2015 1,917 7 View

Is anyone familiar with beta potential of Calcium-alginate nanoparticles ?

23 October 2014 9,694 3 View

How do I remove eukaryotic genomic DNA from PCR fragments?

I have a mixture of a PCR fragment and Salmon Sperm DNA. I'd like to reuse the PCR fragment and get rid of the genomic DNA. Is there a RE similar to DpnI that cuts eukaryotic DNA? Thanks!

27 August 2014 521 4 View

What do you think about the "Editions Universitaires Europeennes"?

Dear all, What is the desired benefit of publishing with the "Editions Universitaires Europeennes" https://www.editions-ue.com/ ? Do you consider it a predatory...

01 January 1970 1,122 20 View

What is the appropriate way to design primers with specific SNPs?

I want to design some primers to characterize differents SNP (Single Nucleotid Polymorphisme) in a simple process using PCR and agarose gel, not for sequencing. What could be the appropriate way...

01 January 1970 9,712 6 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

I have designed reflectarray element and feeding it though Horn Antenna on HFSS. After full wave simulation, I am not getting desired results?

I have designed reflectarray element to generate OAM beams at 5.8GHz and feeding it though Horn Antenna on HFSS. After full wave simulation, i am not getting desired results i.e Radiation pattern...

01 March 2021 9,701 5 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Microstrip antenna design tutorial of HFSS 3D layout module ?

I have designed a 24 GHz rectangular antenna on HFSS 3D model and got desired results while desing the same antenna with same dimensions I am getting resonance frequency at 27 GHz I am...

28 February 2021 4,426 2 View