I am testing a technic for the determination of thiomersal, I use a method with dithizone; I put in a test tube a solution of thiomersal and complete to 1ml with water, then I add the reagents which are:
Acetone, dithizone (diluted in acetone and kept cool) and NaOh.
I vortex and read the absorbance on the spectrophotometer at 538nm.
I have a problem with the spectrophotometer reading, it shows several absorbance values.
I have tried adding EDTA to the mixture to try to eliminate the interference, but the spectrophotometer still shows multiple values.
Does anyone have a solution to this problem? I would like to stabilize this mixture and have only one value displayed on the spectrophotometer to make sure that the results I get are accurate.
Any help would be appreciated
Thank you
Hi Amel,
I can't answer your question and others too will have a problem because it's not clear what you mean by 'it shows several absorbance values' .. (at 538 nm) ..
Do you mean that the values for replicates are not repeatable?
Is the spectrophotometer working well at the selected wavelength?
Paul.
Hello,
I mean that when i place the cell in the spectrophotometer, it shows several values at the same time, fo example : 0,400 .. 0,405 .. 0,390 and it doesn't settle to one exact value.
Are you suggesting that i should change the wavelenght ? If so, wich one should i use ?
Thank you for your answer
I'm suggesting that you put in a solution of something about the same colour that you know is stable and see what happens to find out if the problem is in the spectrophotometer or in your 'solution'.
If the problem is with the solution then it sounds like there may be solids present
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