Can I use standard graph of pure curcumin?
Yes you can.....
better to use HPLC or HPTLC method rather than simple UV.
because OF HIGH ABSORBANCE OF CURCUMIN UV method is too very sensitive.
Solvent play a big role so either dry the extract first or make a calibration curve of standard in same ratio of sovents
Calculate yield directly in percentage yield of curcumin using formula
Wt of curcumin/Wt of raw material X 100
You can calculate the Curcumin % yield using formula: % Yield of the extract = Cx /CY X100
Where Cx = Curcumin weight after extraction process
CY = Curcumin material weight taken for extraction
Shanker Kalakotla Hi, i would like to ask either the weight of sample after extraction process is before or after being remove the solvent by using the rotating evaporator ?
Yield (%)= (W1*100)/W2
Where,
W1= weight of the extract residue obtained after solvent removal
W2= weight of the plant powder.
Or
Yield (%)= (W2-W1/W0*100)
W1= Weight of extract with container
W2= Weight of blank container
W0= Weight of the initial dried sample
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