Sometimes columns and/or detectors alone are working up to 95-98%, but the overall efficiency of the HPLC system/instrument is not appropriate in terms of its utilization or throughput.
Dear Vimal,
Calculating Theoratical plates alone can help you judge the performance of HPLC.
Just perform it, you will be able to have entire validation with the help of this.
Depends what you need for your particular method. Theoretical plates, peak shape, resolution are all measure of system efficiency and performance and depend on pump configuration, dead volume, dwell volume (for Gradient methods), detector settings, column condition, method parameters etc.
System utilization is something else, and has little to do with the performance of the method.
There is no such a thing like "HPLC system efficiency", Columns and detectors are exchangeable components of HPLC, and their efficiency can be evaluated for specific applications. You can perhaps evaluate efficiency of individual stationary components of the HPLC hardware system, for example pumps, gradient system, degasser, injection port, autosampler etc., but again this would be only valid for specific applications.
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