How can I calculate the MDA concentration in leaves (100 mg) after taking absorbance at 532 nm and 600 nm?

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Prakash Kishore Hazam Popular answer

MDA equivalent n mol/ml = [(A532-A600)/155000] x 1000000

L A Piche

I am afraid I have not worked with plant materials in this regard but you might contact Dr Aysel Sari

Prakash Kishore Hazam

Praveen Roylawar

Thank you L Piche for your suggestion...

Hazam PK can you please tell me why we multiplying the formula by 1000000. that is bit confusing me.I'm grateful for your valuable attachment.

You can get it by the pdf attached to my answer

Dr Uma Kant Sharma

Hello Hazam sir can you tell me how to calculate FRAP value of antioxidant substances and also in plasma. I will be greatly thankful to you.

Hell Uma.........hope the attached document can help you

Thanks sir, if i used substrate concentration in molar term then can i express this value in term of µM of ferrous equivalent Fe (II) per µM of sample. or molar term will be used for sample.

Ayat Rezaei

MDA equivalents (nmol.mL-1) = (A532-A600/157000)1000000