I find it can be detected by CV technique but these authors don't give details, so please help me if you know, thanks.
The paper you attached describes how well an enzyme (in that case PQQ) is immobilized to SAMs and further detects this by using the cofactor of the enzyme. It seems to be a roundabout way to go about it and involves enzyme kinetics which would make things harder.
It would be more facile to find a molecule that is easy to see electrochemically and relatively easy to link it to SAMs and then do voltammetry and calculate how much of that molecule is available. Probably should use excess to make sure that all the SAMs are connected to your chosen molecule.
Article Discerning the Redox-Dependent Electronic and Interfacial St...
this is an example with Fc.
Hi Fang Xie
Cyclic voltammetry can be used to look at the density of a SAM on a Au surface by looking at the electrochemical desorption of the SAM from Au. The basic idea is that you form the SAM on the Au, then run a CV of this to negative potentials, which results in a peak due to the stripping of the SAM from the Au. The charge of this peak can then be used to determine the coverage of the monolayer. This paper should give you more information: https://www.sciencedirect.com/science/article/abs/pii/002207289185271P
That's helpful, thank you! The SAM in my experiment is DNA linked with methylene blue (MB). I think the coverage can be calculated by the ratio of Q to (nFA), Q means the charge and can be measured by integration of cathodic or anodic peak of MB, n here is 2 and A is attributed to the geometry area of electrode, F is the Faraday constant, 9.65*10^4 C/mol@Mihaela Apetrei
Article Chemical Equilibrium-Based Mechanism for the Electrochemical...
Fang Xie Good luck :)
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