How can I Calculate the adequate number of Bacterial strain of Petri Dish during Antibacterial activity study execution?

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Adam B Shapiro

It might be a good idea to use the guidelines for disc diffusion assays that are approved by the Clinical and Laboratory Standards Institute (CLSI).

An example from the World Health Organization of a highly detailed standard operating procedure (SOP) following these guidelines for susceptibility testing by disc diffusion assay is attached:

http://www.antimicrobialresistance.dk/data/images/protocols/sop_dd_2010gfnlab002_v2.pdf

Md Sarwar Hossain

Thank You very much for your reply.

Matěj Božik

http://www.eucast.org/ast_of_bacteria/