Hi, Dear All, I got some results of my master thesis (atropine and scopolamine detection in contaminated millet with Datura seeds) with HPLC-MS/MS. Now, I want to calculate LOD and LOQ. I know it is 3 times and 10 times of S/N ratio, respectively. My question is how can I report one exact number for LOD and LOQ. Is it an approximate number?!From which chromatograms can I calculate these numbers?The only way is using a software e.g. MultiQuant to calculate?! I am confused about this.
Thank you for your time in advance. Regards,
Saman