Orbitrap Fusion FT keep getting disconnected?

Hi, I been having a problem with the Orbitrap Fusion for quite a while and it has been getting more and more frequent, which is very frusttrating. The fusion FT would get disconnected suddenly...

22 January 2021 1,235 4 View

What is the basis set to optimize ZnO using GaussView for DFT calculation study?

Greetings, I have a bit of problem in setup the basis set of my DFT study for ZnO.. As you can see in the image attached below, I use B3LYP 6-31G (d,p) for geometrical opt. of ZnO.. But sadly, I...

13 January 2021 7,508 4 View

How does titanium dioxide (TiO2) as a coating's component enhance the coating performance in terms of anticorrosion??

Greetings, Recently, I was asked by a researcher how does TiO2 enhance the coatings polymeric structure making it able to reduce corrosion reaction by preventing the penetration of aggressive...

02 January 2021 5,257 5 View

What are the suitable amplitude of sinusoidal perturbation signal to analyzed stainless steel 316L?

Greetings researchers, I want to ask for your suggestion in regards to what is the suitable amplitude of sinusoidal perturbation signal to analyzed the conditions of bare and coated stainless...

07 December 2020 7,699 7 View

How to improve DNA extraction using TRIzol and chloroform?

02 December 2020 1,919 1 View

What is the solubility of crude extract of plants in water?

Greetings all, To my limited understanding, the crude extract of plants obtained using solvents such as ethanol and methanol are usually insoluble in water. Is this correct? If it is soluble in...

02 December 2020 1,024 6 View

Does the concentration of the fluorescent compound affect the fluorescent reading on a 96-well plate?

I am trying to detect the fluorescent level of the extracted RNA sample on a 96-well plate. After extraction, I dilute the RNA sample in 20ul solvent. Can I load the 20ul RNA sample directly to...

30 November 2020 2,616 2 View

Any available international research grant in this COVID-19 year?

Dear researchers, I am a researcher from a university in Malaysia. I know that we all are having a tough situation in this pandemic year. I just posted this to know if there is any possible...

24 November 2020 8,759 3 View

In your opinion, what are the mechanism performed by corrosion inhibitor in a coating for corrosive medium?

Greetings researchers, There are quite a number of studies has been performed to analyze the effects of corrosion inhibitor when incorporated in a coating (epoxy, alkyd and etc) for the...

23 November 2020 6,622 10 View

For a marine coating, how much does its mechanical strength needs to be?

Greetings to all researchers, First and foremost, I would like thank all of you for answering this question. So, my question is quite general I guess, for a standard marine coating with good...

08 November 2020 8,880 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is there a deep reason for using the squared modulus of the wave function as the probability density of the existence of the particle?

I think we were all surprised at the first time we got to know quantum mechanics that the squared modulus of the wave function is the probability density of the existence of the particle? The role...

28 February 2021 6,282 9 View

What could be reason for getting such type of graph for complex impedance in case of polymer blend films?

Its effect appears in real part of ac conductivity also. Can anyone suggest any reason behind it?

26 February 2021 3,487 3 View

How can I stain the somatodendritic part of inhibitory neurons in a hippocampal culture with high specificity?

I would like to distinguish the somatodendritic part of the inhibitory neurons in a hippocampal culture (where we expect to have 5-10% of GABAergic neurons). What are the best antibodies and...

23 February 2021 6,756 1 View

Resistivity of PVD TiNx thin films vs. N2 flow?

In the PVD of TiN thin films (~100A), I've found the resistivity of the TiN films dropped from ~100 to 60um*cm with N2 flow increasing up to the metallic mode ending point; then the resistivity...

21 February 2021 1,864 2 View

How do I add a udf to Ansys fluent and does it need to be compiled before hand ?

I have a Define_CG_Motion udf written in c where do I add it to my Ansys fluent project and does it need to be compiled before that?

21 February 2021 9,347 3 View

The PC12 cell line characteristics?

Hello dear scientists, Does the undifferentiated PC12 cell line in terms of chromosome characteristics, proliferation, and drug reactions have the characteristics of cancer cells? Where is it...

21 February 2021 8,945 3 View

How to implement boundary conditions in Abaqus contact problems?

Hello I am trying to simulate crawling motion of a bilayer beam using Abaqus. Kind of similar to this: https://youtu.be/os-Z3ucoFpA I am using multiphysics enabled (diffusion, deformation,...

20 February 2021 2,916 1 View

What are alternatives for B27+ Supplement in neuronal cell culture?

Hello everybody In our iPSC-derived neuronal culture we use Maturationmedium, supplemented with B27+. As Thermo Fisher run out of stock and can deliver earliest in May, I wanted to ask whether...

16 February 2021 3,348 1 View

Unusually Busy Raman Background?

Hello, I am observing a nearly periodic background spectrum for a wide variety of SERS substrates. I see this response (attached below) for bare Au films, Cu films, and Au nanoparticles on...

15 February 2021 9,922 5 View