Patients with chronic kidney disease could get COVID-19 vaccine?

04 February 2021 278 4 View

Post doctoral positions ?

I am looking for a post doctoral position in the field of optical thin films and associated area. Kindly let me know if you come across any active advertisement in near future where i can apply.

04 February 2021 9,622 4 View

What will be the impacts of post covid-19 stress on working nurses?

The health and well-being of nurses are essential to the quality of care that they provide for people and communities. The Covid-19 pandemic has put them under unprecedented pressure. The impact...

26 January 2021 2,440 20 View

How should I prepare my tissue sample for biodistribution study?

I want to evaluate the biodistribution of an elastin-based nanoparticle drug in tumor, heart, liver, and kidney for the first time. Can anyone give me any idea about how to prepare my tissue...

04 January 2021 3,661 3 View

What are the alternative to Rotary Evaporation?

I want to remove solvent to obtain a solid . i would like to remove solvents such as THF, TEA, carbon tetrachloride . the obtained solid which is a Phosphorus ester. What could be an suitable...

04 January 2021 1,220 1 View

How fishery by-products/fish waste can be utilised as efficiently, cost-effectively and eco-friendly?

Fishery by-products utilisation in the industry with minimum cost and low carbon emissions.

29 December 2020 1,752 11 View

How to download thesis from Krishikosh or Sodhganga?

Recently I have found some thesis in Krishikosh but unable to download the pdf files. Is anybody knows how to download these files?

25 December 2020 2,706 2 View

Will the new COVID-19 strain, identified in England, affect the vaccine?

According to the CDC, the UK variant has an unusually large number of mutations -- 14 changes and 3 deletions in its genetic code that impact the building blocks of its proteins. This virus has 17...

23 December 2020 375 15 View

Cite score or Impact factor: which one is more reliable to rate the journal quality?

There are so many good journals having scopus indexed but no impact factor, so is it time to look for other measures like cite score?

10 December 2020 2,797 3 View

Is there any database of UK based advertisements? I am specifically looking for anti-counterfeiting ads. Any suggestion?

I am looking for print/tv/social media/outdoor anti-counterfeiting advertisements targeted to British consumers.

23 November 2020 7,950 2 View

How can I make stacked bar graph with scatter plot?

I want to make a stacked bar graph with showing each data value as dots. I tried with graph pad prism, version 6, but could not succeed. Does anyone have tried before making such graphs?

01 March 2021 9,383 1 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

Negative result on natural product sample cholinergic assay?

i have been doing cholinergic assay on natural product and for some of the samples, i get negative value for the inhibition activity. can anyone help me explain the meaning of the negative value...

17 February 2021 4,118 1 View

What is the proper in vitro culturing method to get information about the activation of innate immune cells by VLPs?

Dear All, I am interested in studying the response of immune cells against virus-like particles (VLPs). I used the PBMC in vitro assay and treat it with the VLP using PHA as a positive ctrl while...

16 February 2021 1,337 2 View

Why does flow deformation occur at the test line and control line on nitrocellulose membranes (LFIA)?

I use 1% BSA in 10 mM phosphate buffer (pH 7.4) solution when striping my nitrocellulose membranes with antigens and antibodies. However, I always observe deformed flow when my sample or running...

16 February 2021 4,224 3 View

Can the viability assays (ex: MTT/CCK-8) measure cell proliferation? or are they solely viability assays?

I want to conduct a proliferation assay to test a chemotherapy combination on a cancer cell line. At first, I thought about the MTT assay. But now, all sources are saying that these do not measure...

14 February 2021 3,142 7 View

GST assay in microplate?

I'd like to know if anyone can provide me with a protocol for traditional GST enzymatic assay (with GSH and CDNB as substrates) in a 96 well microplate, for cell line and/or C.elegans...

11 February 2021 1,328 3 View