From my previous experiment, I have calculated X cells/ml and Y number of total cells from a T-25 flask.Like others, first I trypsinized the cells from T-25 flask, counted in a haemocytometer and got the value of cell seeding density and total number of cells.How can I convert the value for a 6-well, 12-well and 96-well plates?Thank you
Depending of your needs and experiment design just select appropriate plating density f.e. 5000 or 10000 cell per cm2 will be good for long experiments with big number of cell doublings. If you want to use f.e. 20 % of monolayer then just calculate dilution from known flask area and resulting cell concentration.
It depend of plate type, but the usual area for 6-well is around 9 cm2, 12-well - around 4 cm2, 24-well - around 2 cm2 and 96-well plates around 0.3 cm2
Take a look link below and you will find some additional useful information on topic:
https://www.thermofisher.com/by/en/home/references/gibco-cell-culture-basics/cell-culture-protocols/cell-culture-useful-numbers.html
Wellcome. The button "Recommend" was created not only as analog of "like button" but also as a scientific-like parameter of comment value ;)
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