How can I calculate cell counts using Image J?

05 September 2022 0 7K Report

I'm working on a 3D spheroid model for thyroid cancer. I want to do photo edits and call counts via Image J. Is there anyone who can help with this?

Badges
Science topic

Similar topics
Education
Students

More Ezel Bildik's questions See All

Is there a standard protocol you use for Colony Formation Analysis?

Hello. I will do Colony Formation analysis for my master's thesis. I'm trying to create a standard protocol for Clonogenic assay or colony formation assay. I would appreciate it if you could...

29 April 2024 3,481 0 View

Is there a standard protocol you use for Colony Formation Analysis?

Hello. I will do Colony Formation analysis for my master's thesis. I'm trying to create a standard protocol for Clonogenic assay or colony formation assay. I would appreciate it if you could...

20 April 2024 9,197 0 View

Steps for the isolation of mitochondria from cancer cells?

We would like to obtain mitochondria from cancer cell lysates. However, we do not have a clear protocol. Additionally, we do not have the kit to enable mitochondria isolation. Do you have any...

06 November 2023 6,117 2 View

What are the methods or kits used to measure mitochondrial oxygen respiration in cancer cells?

Hi there! I am working with hepatocellular carcinoma cells. I want to study oxygen consumption in these cells. Is there a kit or a procedure I can use other than the XF Cell Mito stress kit?

30 July 2023 5,957 2 View

How can we look at autophagy markers from tissue lysates apart from western blot and immunohistochemistry?

Hi there, After constructing a metastatic cancer model, I would like to look at autophagy markers in C57BL/6 type mice. Which method should I choose other than WB and IHC? Thanks

01 March 2023 2,746 2 View

How can I adapt the cell culture IC50 dose to the animal?

The agent I will use does not have a suitable dose for the animal. I want to apply the dose I found in cell culture.

05 December 2022 7,808 3 View

How can IC 50 calculate using excel?

what is the right way of determining IC50 using microsoft excel?

24 January 2016 6,580 71 View

Is there study about growth hormone gene expression in salmon trout?

Is there an article about the isolation from blood or brain tissue? Can I isolate the whole brain tissue?

21 June 2015 4,089 1 View

The half life of mRNA is very short. How do the results of mRNA reflect as true the gene expression?

27 January 2013 5,094 9 View

Can you suggest reliable sources defining "3D mesh" and "3D city models"?

Dear fellow researchers, I am currently working on a paper where I need to provide a reliable reference that defines and distinguishes between 3D mesh models and 3D city models. Although I am...

06 August 2024 9,986 2 View

What are the shear and normal stiffness values of an LLDPE liner in 3D numerical modeling of a stockpile?

I am seeking experimental or applicable data for the liner (LLDPE) interface in FLAC3D numerical modeling of a large stockpile. Could you please recommend suitable references? The preferred data...

05 August 2024 3,665 0 View

Radiogenomics Cancer Research Challenges?

what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...

03 August 2024 5,828 4 View

CAD File of human's & rat's respiratory airways ?

Dear all, I am working on particle deposition in human's & rat's respiratory airways using CFD and I am looking for the 3D CAD file for my simulations (STEP or IGES format). If somone has such...

29 July 2024 1,092 2 View

What is your major research area?

My major research area is clinical research and basic research of thyroid and parathyroid.

26 July 2024 3,639 14 View

Cell optimization in 1D Nanotubes?

Dear All, I use Quantum Espresso. I want to optimize the structure of the carbon nanotube using the vc-relax. I got confused about how to fix cell_dofree here. For 3D, cell_dofree = 'all' or...

22 July 2024 5,708 0 View

For a case-control study for Breast Cancer incidents in a particular County in USA, what data can be used as Control? E.g. colorectal cancer?

The County health department is providing Breast cancer incident data. If colorectal cancer cases could be used as control, then we can get respective data from health department as well.

22 July 2024 9,954 4 View

About Machine Learning for Automated Diagnosis of Skin Cancer with Dermoscopy Images ?

Machine Learning for Automated Diagnosis of Skin Cancer with Dermoscopy Images

21 July 2024 5,471 0 View

Fixation Problem with cancer cells?

Hello everyone, I work with polyacrylamide hydrogels, which I fictionalise with sulfo-sanpa and collagen I to then plate lung cancer cells. The problem is that I lose a significant amount of...

19 July 2024 3,906 0 View

Does anyone notice the tumor regression impact of Hepa1-6 cells in HCC orthotopic mice model?

To establish an orthotopic HCC model, C57BL/6 mice (male, 6-8 weeks) were orthotopically implanted with 3x106 Hepa1-6 cells (Over-expressed with a human gene) in 20uL Matrigel into the left lobe...

18 July 2024 9,177 2 View