What are the methods or kits used to measure mitochondrial oxygen respiration in cancer cells?

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Amadeo M Parissenti

Hi Ezel: In terms of measuring oxygen consumption in tumour cells, I would recommend the CCK8 assay, which measures cellular dehydrogenase activity that comes principally from mitochondrial respiration. The dehydrogenases are oxidoreductase enzymes that catalyze the oxidation of a substrate through the transfer of hydrogen (hydride) to an acceptor (or co-enzyme). In the case of the CCK8 assay, the acceptor would be WST-8, which is then transformed into a formazan salt. Since this is an oxidation reaction that occurs in oxidative phosphorylation, I believe it would be a good measure of oxygen consumption.

Details can be found from one of the suppliers of the CCK8 kit (Sigma Aldrich): https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/381/017/96992dat.pdf

Hope this is helpful.

Amadeo

Ezel Bildik

Amadeo M Parissenti Thank you for your reply. I'm going to do research on that.