Is there a standard protocol you use for Colony Formation Analysis?

Ezel Bildik @Ezel-Bildik

21 April 2024 0 9K Report

Hello. I will do Colony Formation analysis for my master's thesis.

I'm trying to create a standard protocol for Clonogenic assay or colony formation assay.

I would appreciate it if you could share your knowledge on this subject.

Thanks in advance.

Important questions:

What to use when fixing cells before imaging? (such as formaldehyde, praformaldehyde or methanol)

Is there a specific temperature for the solution?

How long is the incubation period of the solution?

Are you viewing the fixed cells with a microscope or a special device?

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