22 September 2017 3 871 Report

for LDH enzyme assay, after addition of my test sample in the assay reagent, i have recorded decrease in A340nm for approximately 5 mins. i have a data of decrease in OD for 5 mins at interval of 1 min. 

how can i calculate  ( ΔA340/min test  ) ?

Units/ml enzyme= (ΔA340/min test  - ΔA340/min blank) *2.7*df / 6.22*0.05

2.7 =total volume of assay

6.22= extinction coefficient of NADH

0.05= volume of sample.

and how to obtain ΔA340/min using the maximum linear rate for both the test and blank?

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