How can I analze compostion of SEBS/PP blend via FTIR or DMA? Can I also determine relative amount of strenics to mid block to PP?

More Zehra Kalkan - Sevinc's questions See All

Why yeast transformation is not working?

I have been trying to delete one copy of an essential gene from a diploid in fission yeast. I am using PCR based homologous recombination method and primers are designed from Bahler's lab website....

12 May 2024 1,274 4 View

Is this a contamination in cell culture?

I saw these structures forming in my cell culture. Does anyone know what these are? Have you experienced such a situation before?

04 February 2024 3,791 6 View

What could this be?

We started to see such objects in the flasks containing our canser cell lines? What could these be? Could the cells have been contaminated?

14 September 2023 5,953 0 View

Is this a contamination?

When studying HT-29 colon cancer cells, we observed such a structure in culture. It has a distinct green color. What could it be?

12 September 2023 9,971 0 View

Why are there different T2A sequences?

There are different T2A sequences available although all of them share the same protein sequence. Are they codon optimized according to species?

29 July 2022 3,981 0 View

How to measure DNAse I stock solution enzyme activity?

We have DNAse I stock solution (obtained commercially, still lyophilized, should be dissolved in 10 mL according to supplier) and it has been sitting in a closet way too long (nearly 10 years)....

09 June 2022 2,945 0 View

Simple and free Antisense oligonucleotide designing tools?

We want to design antisense oligonucleotides for suppressing the gene of interest. The databases or related literatures found are either old or irrelevant. We are new in this field. Are there ASO...

22 March 2022 5,469 0 View

Is there a way to isolate shRNAs from other RNAs -including mirna- ?

We are planning to transfect HEK293T cells with plasmid that carries shRNA sequence against the gene of interest, grow a large batch and let them produce shRNAs, and then isolate shRNAs. But the...

10 March 2022 1,781 1 View

How to store shRNA-transfected cells?

We want to inhibit gene expression via shRNA in prostate cancer cell lines and then store them for a while for further experiments (apoptosis, proliferation, qRTPCR etc.). I know that it is...

04 January 2022 5,726 2 View

Does anyone used ONT (nanopore) system for Single cell sequencing instead of illumina?

Hi. We are planning to do a single-cell RNA seq in combination with an ONT system for sequencing instead of Illumina. So is there anyone who has done it before? There are a few steps in the...

09 November 2021 5,315 4 View

How top dressing of nitrogenous fertilizers can be done in mulched wheat and rice crops and how should FYM/compost be applied in zero till wheat?

02 June 2024 3,836 3 View

UV polymerisation of styrene?

Hi everyone, I am attempting to polymerize styrene using UV initiators, specifically BAPO, even at a concentration of 5 wt%, I am experiencing limited success with this approach. Has anyone...

30 May 2024 2,509 4 View

Mechanism of copolymer?

Kindlly provide links or literature about the mechanism of free radical copolymerization of styrene and methacrlate, kinetics and rate equation

27 April 2024 404 2 View

What is the relationship between hald peak width of the Tan (delta) peak (in DMA) of a polymer and the network structre of the polymer?

A DMA graph of a thermoset polymer gives the storage modulus graph, loss modulus graph and a Tan (delta) graph. Tg of the polymer can be either Storage onset temperature or the Tan (delta) peak...

24 April 2024 1,176 3 View

Are you a researcher working on Sustainable Packaging Manufacturing topics?

The global push towards sustainability has sparked significant interest in the development of eco-friendly packaging solutions that minimize environmental impact throughout their lifecycle....

21 April 2024 561 5 View

How to do a partial budget analysis for research on integrated fertilizer application ( mineral with compost application)?

The added compost may not mineralized in the incorporation year. Hope it may mineralize fully in more than two years. So how to calculate the cost of compost for one (incorporation) or divide the...

20 March 2024 7,327 2 View

What is the role of microorganisms in the nutrient cycle and what microorganisms convert agricultural wastes to compost?

05 March 2024 353 2 View

How to measure the DMA peak of Propolyene?

Hello every expert, When I did the DMA expertment for propolyene, I can't not get the normal spectrum. I intended to find the tg peak of PP, but it was not easy to have the beautiful peak in this...

22 February 2024 7,216 0 View

Why is DMA data not continuous at low temperatures?

I am performing a temperature sweep from -100 to 100C at a 3deg/min rate to determine Tg of elastomer samples using the DMA 850 model. The data doesn't begin collecting at -100C (attached data...

19 February 2024 8,322 3 View

Comparing 1 ton fresh vs. composted residues on soil carbon sequestration?

Dear ResearchGate Community, I am conducting an analysis to compare the carbon sequestration potential of applying 1 ton of fresh organic residues directly to soil versus the application of 1 ton...

13 February 2024 10,012 6 View