Hi everyone,

To combine multiple runs for analysis, you need to use the Gene Study option. For this go to the program's main screen. Select File>New>Gene Study. On the Gene Study screen, select File>Add Data Files. 

You can analyse the data on the 'Study Analysis' tab

You can refer to the following links to obtain certain interesting informations:-

http://www.bio-rad.com/en-in/product/cfx-manager-software

You can also download pdf of the file attached herewith.

Hi, Alice. Did you already got the answer? If yes, it would be really nice of you to share that because I am searching for the same answer as well.

Hi Alice. Did you find any solutions to your query. I am using the same version of CFX and have the same question.

Hi everyone,

To combine multiple runs for analysis, you need to use the Gene Study option. For this go to the program's main screen. Select File>New>Gene Study. On the Gene Study screen, select File>Add Data Files. 

You can analyse the data on the 'Study Analysis' tab

Hi everyone,

I am using the same software for Gene study. My question is after you set youe control sample(untreated group) when you come to the result tab the p value is calculated differently than a normal 2-tailed ttest when you do in excel. Does anyone know why?

On the software if you go to Help>Search >P value>Data analysis formulas> Pvalue you can see how the software calculates.

Thank you!

Hi,

As mentioned by Fatema, you use the Gene Study option for this. I would just like to add that you need to make sure that your sample and target names are exactly the same between the data files that you want to merge into a Gene Study. Also, it is worth checking the target stability value to determine the stability of your reference targets used in the runs. It is highly recommended to use at least 2 different reference targets.

Good luck to all!